The optimum oxygen level in hypoxic culture conditions of ligament derived stem cells: experimental research

Rhatomy, Sholahuddin and Utomo, Dwikora N. and Prakoeswa, Cita R. S. and Suroto, Heri and Tinduh, Damayanti and Notobroto, Hari B. and Arfian, Nur and Rantam, Fedik A. and Mahyudin, Ferdiansyah (2023) The optimum oxygen level in hypoxic culture conditions of ligament derived stem cells: experimental research. ANNALS OF MEDICINE AND SURGERY, 85 (6). pp. 2689-2694. ISSN 2049-0801

[thumbnail of The_optimum_oxygen_level_in_hypoxic_culture.pdf] Text
The_optimum_oxygen_level_in_hypoxic_culture.pdf - Published Version
Restricted to Registered users only
Available under License Creative Commons Attribution.

Download (733kB) | Request a copy

Abstract

Background:The hypoxic condition is a physiological norm for various stem cells. The natural microenvironment contains lower oxygen pressures. Recent studies reported significant increases in the cultured cells' proliferation in the presence of a low oxygen pressure. Objective:This study aimed to investigate the optimum oxygen level for rabbit cruciate ligament fibroblast cells culture and Ligament Derived Conditioned Medium/LD-CM (Secretome) preparation in vitro. Materials and methods:Fibroblasts were isolated from the cruciate ligament of the rabbit's knee. Cultured of rabbit cruciate ligament Fibroblast Cells (fifth passage) were assigned to the slight (5% O-2), middle (3% O-2), and severe hypoxia (1% O-2) groups and the normoxia (21% O-2) group. Measurement of growth factors: TGF-& beta;1, PDGF, FGF, and VEGF in LD-CM (Secretome) used an enzyme-linked immunosorbent assay. Results:The highest number of cultured cells were in the 5% O-2 group compared to the normoxia, 1 and 3% groups. The hypoxia 5% group also had increased productions of PDGF, FGF, and VEGF proteins in LD-CM (secretome) compared to the 1, 3%, and normoxia groups. TGF-& beta;1 production was slightly higher in the 3 group than the 5% group. Conclusion:The hypoxic precondition of 5% oxygen was the optimum condition for ligament culture and ligament derived conditioned medium (secretome) preparation in vitro.

Item Type: Article
Uncontrolled Keywords: hypoxia; ligament derived conditioned medium; normoxia; proliferation; secretome
Subjects: R Medicine > RL Dermatology
Divisions: Faculty of Medicine, Public Health and Nursing > Public Health and Nutrition
Depositing User: Yuliawati Dahniar Dahniar
Date Deposited: 14 Aug 2024 02:19
Last Modified: 14 Aug 2024 02:19
URI: https://ir.lib.ugm.ac.id/id/eprint/3620

Actions (login required)

View Item
View Item