PGV-1 permanently arrests HepG2 cells in M phase and inhibits liver carcinogenesis in DMH-induced rats

Novitasari, Dhania and Kato, Jun-ya and Ikawati, Muthi’ and Putri, Dyaningtyas Dewi Pamungkas and Wulandari, Febri and Widyarini, Sitarina and Zulfin, Ummi Maryam and Salsabila, Dhiya Ulhaq and Meiyanto, Edy (2023) PGV-1 permanently arrests HepG2 cells in M phase and inhibits liver carcinogenesis in DMH-induced rats. Journal of Applied Pharmaceutical Science, 13 (08). pp. 204-211. ISSN 2231-3354

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Abstract

Pentagamavunone-1 (PGV-1) has been reported to eliminate cancer cell progression in the breast, blood, and colon.
The current approach evaluates its antiproliferative effects and cellular activity against hepatocellular carcinoma cells
(HCC). We used the HepG2 cells as an in vitro model for HCC, and PGV-1 was tested for its effects on cell viability,
cell cycle modulation, senescence induction, reactive oxygen species (ROS) generation, and cell migration. Moreover,
the ability of PGV-1 to prevent liver carcinogenesis was tested in 1,2-dimethylhydrazine- (DMH-) induced rats. PGV-1
irreversibly inhibited cell proliferation via mitotic arrest and cellular senescence. The ROS production was enhanced
during the earlier hours of incubation with the compound. Later, PGV-1 significantly delayed the HepG2 cell migration
and invasion. Furthermore, PGV-1 prevented steatohepatitis upon DMH administration and drastically reduced the
Ki-67 expression in DMH-induced rat liver, indicating its ability to suppress aberrant liver cell proliferation. These
findings add to the evidence that PGV-1 could be further developed pharmaceutically as a candidate for cancer therapy
with a specific target on mitosis.

Item Type: Article
Uncontrolled Keywords: Curcumin analog (PGV-1), liver cancer, mitotic arrest, anti-carcinogenesis.
Subjects: S Agriculture > SF Animal culture
Divisions: Faculty of Veterinary Medicine
Depositing User: Erlita Cahyaningtyas Cahyaningtyas
Date Deposited: 11 Sep 2024 03:06
Last Modified: 11 Sep 2024 03:06
URI: https://ir.lib.ugm.ac.id/id/eprint/3676

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