Kusumawati, Asmarani and Satrio, Faisal Amri and Indriastuti, Rhesti and Rosyada, Zulfi Nur Amrina and Pardede, Berlin Pandapotan and Agil, Muhammad and Purwantara, Bambang (2023) Sperm Head Morphology Alterations Associated with Chromatin Instability and Lack of Protamine Abundance in Frozen-Thawed Sperm of Indonesian Local Bulls. Animals, 13 (15): 2433. pp. 1-13. ISSN 20762615
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Abstract
This study aimed to analyze various alterations in the morphology of the sperm head and its association with nucleus instability and insufficient sperm protamine. Frozen-thawed semen from twenty local Indonesian bulls was used for all stages in this study. The results of sperm head defect assessments are used for bull grouping, high (HD) and low (LD). Sperm DNA damage was assessed using Acridine Orange and Halomax. The PRM1 protein abundance was carried out using an enzyme immunoassay, while PRM1 gene expression was carried out using the RT-qPCR. PRM deficiency was
performed using CMA3 . Several kinds of sperm head defects in the HD were significantly higher (p < 0.05) than in the LD bulls. Sperm DNA damage showed a significant (p < 0.05) difference between the HD and LD bulls. PRM1 abundance was significantly (p < 0.05) decreased in HD bulls. PRM
deficiency was significantly (p < 0.05) higher in HD bulls than in LD bulls. PRM deficiency in bulls correlated significantly (p < 0.01) with sperm head defects, DNA damage, and PRM1 abundance. The lack of sperm protamine might affect the sperm nucleus’s stability and induce morphological alterations in the sperm head.
Item Type: | Article |
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Uncontrolled Keywords: | CMA3; local Indonesian bulls; protamine deficiency; PRM1 abundance; sperm DNA damage; sperm head defects |
Subjects: | S Agriculture > SF Animal culture |
Divisions: | Faculty of Veterinary Medicine |
Depositing User: | Erlita Cahyaningtyas Cahyaningtyas |
Date Deposited: | 11 Sep 2024 02:53 |
Last Modified: | 11 Sep 2024 02:53 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/3677 |