The effect of water content and carbon source addition on lipase production from Aspergillus aculeatus Ms.11 using spent coffee ground

Yuliyanti, A. and Manullang, M.C.D. and Ilmi, M. (2021) The effect of water content and carbon source addition on lipase production from Aspergillus aculeatus Ms.11 using spent coffee ground. In: 4th International Conference on Biosciences (ICoBio 2021), 11-12 August 2021, Bogor.

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Abstract

Lipase is one of the industrially important enzymes, however, the production needs costly substrate. To overcome the problem, we developed an effective medium formulation to produce lipase from indigenous lipolytic mould Aspergillus aculeatus Ms.11 using spent coffee ground. We observed the effect of additional glucose and olive oil, and the influence of water content on lipase production. The experiments were done using the Solid-State Fermentation (SSF) method for 7 days. The results show that optimum lipase production on substrates with additional glucose and olive oil observed on day 4 with lipase activity of 16.296 U/mL and lipase productivity of 150.32 U/g/day. The optimum water content from the results is 50. The highest lipase activity obtained using the water content is 291.80 U/mL, while the highest lipase productivity is 106.32 U/g/day. The results showed that water content as well as the addition of glucose and olive oil, affects lipase productivity of Aspergillus aculeatus Ms.11 on spent coffee ground. Further studies to optimise the production condition are suggested. © Published under licence by IOP Publishing Ltd.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Cited by: 1; Conference name: 4th International Conference on Biosciences, ICoBio 2021; Conference date: 11 August 2021 through 12 August 2021; Conference code: 175800; All Open Access, Gold Open Access
Subjects: Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Biology > Undergratuate Program in Biology
Depositing User: Sri JUNANDI
Date Deposited: 07 Oct 2024 00:52
Last Modified: 07 Oct 2024 00:52
URI: https://ir.lib.ugm.ac.id/id/eprint/5027

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