Isolation and identification of nitrifying bacteria from tilapia (Oreochromis sp.) pond in Sleman Yogyakarta Indonesia

Aswiyanti, I. and Istiqomah, I. and Isnansetyo, A. (2021) Isolation and identification of nitrifying bacteria from tilapia (Oreochromis sp.) pond in Sleman Yogyakarta Indonesia. In: The 4th International Symposium on Marine and Fisheries Research.

[thumbnail of Aswiyanti_2021_IOP_Conf._Ser.__Earth_Environ._Sci._919_012054.pdf] Text
Aswiyanti_2021_IOP_Conf._Ser.__Earth_Environ._Sci._919_012054.pdf
Restricted to Registered users only

Download (699kB) | Request a copy

Abstract

This research aims to isolate and identify autochtonous nitrifying bacteria from tilapia pond in Sleman Yogyakarta Indonesia for future application in aquaculture practices in the region. Bacteria were isolated using a nitrification medium. Bacterial characterization was carried out by non-pathogenic test to tilapia (Oreochromis sp.), and nitrification activity test in a single bacterial fermentation medium for 9 days. Bacterial identification was carried out based on the colony and cell morphologies, biochemical tests, and molecular analysis using the 16S rRNA and gyrB genes. A total of 15 isolates of nitrifying bacteria were obtained. Four non-pathogenic isolates obtained the highest nitrification activity on the sixth day of incubation, with nitrate production of 17.26-21.54 ppm. Two selected bacteria, isolates A2 and A3, have colony morphology that is milky white, smooth surface, circular shape, entire edge, and convex elevation. Both bacteria are short rods, Gram-negative, non-motile, produce catalase, fermenting glucose, sucrose, and lactose, and do not produce oxidase, ornithine decarboxylase, indole, and H2S. Molecular analysis showed that the two isolates had the highest similarity (99.28 and 99.34) to Klebsiella spp.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Library Dosen
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Faculty of Agriculture > Department of Fisheries
Depositing User: Sri JUNANDI
Date Deposited: 30 Sep 2024 06:00
Last Modified: 30 Sep 2024 06:00
URI: https://ir.lib.ugm.ac.id/id/eprint/5040

Actions (login required)

View Item
View Item