Identification of restriction enzyme in the FSHR gene of indonesian local cattle

Prihandini, P. W. and Primasari, A. and Luthfi, M. and Pamungkas, D. and L Sari, A. P. Z. N. and Dina, T. B. and Maharani, D. (2021) Identification of restriction enzyme in the FSHR gene of indonesian local cattle. In: 2nd International Conference on Animal Production for Food Sustainability 2021.

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Abstract

The restriction enzyme is important for genotyping using the PCR-RFLP technique. Therefore, this study aims to identify the restriction enzyme mapping in the partial sequence of the follicle-stimulating hormone receptor (FSHR) gene in Indonesian local cattle. A total of 29 samples sized 306 bp, were aligned with Genbank sequence acc no. NC032660, resulting three polymorphic sites, namely g.193G>C, g.227T>C, and g.275A>C. Furthermore, the restriction mapping analysis using the NEBcutter program V2.0 showed that no enzyme recognized the SNP g.275A>C, while the SNP g.193G>C and g.227T>C were identified by the AluI and MscI enzymes, respectively. The AluI enzyme cuts at two positions (193 bp and 243 bp) in the G allele sample producing three fragments namely 50 bp, 63 bp, and 193 bp, meanwhile, in the C allele, the AluI cuts only in position 243 bp, hence, the fragment products are 63 bp and 243 bp. In contrast, the MscI enzyme was only recognized in the T allele, producing fragments sized 77 bp and 229 bp but failed to identify the restriction site along with the PCR products in the C allele. Based on the results, the SNPs (g.193G>C and g.227T>C) and restriction enzymes (AluI and MscI) are applicable for genotyping local Indonesian cattle using the PCR-RFLP technique in future studies.

Item Type: Conference or Workshop Item (Paper)
Additional Information: Library Dosen
Uncontrolled Keywords: FSHR gene, restriction enzyme, Indonesian local cattle
Subjects: S Agriculture > SF Animal culture
Divisions: Faculty of Animal Sciences > Department of Animal Breeding and Reproduction
Depositing User: Sri JUNANDI
Date Deposited: 04 Oct 2024 03:55
Last Modified: 04 Oct 2024 03:55
URI: https://ir.lib.ugm.ac.id/id/eprint/5087

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