Survival ability of Bacillus cereus LS2B in the presence of tannery wastewater

The leather industry's production activity affects liquid waste containing a high amount of organic and chemical compounds. This study aims to determine Bacillus cereus LS2 B's survival ability in the medium with the presence of fresh untreated tannery wastewater. The growth characterization was made and observed in the agar medium with 0; 25; 50; 75 and 100 tannery wastewater. Growth profile in a liquid medium was observed with the addition of 0; 25; and 50 tannery wastewater. The survival ability of Bacillus cereus LS2B was observed by a visual colony formed in the agar medium, the optical density (OD600) of cell bacteria in the liquid medium, and the cell viability. The growth of Bacillus cereus LS2B could not be confirmed at both solid and liquid medium with tannery wastewater more than 25. The survival ability and cell activity were observed in the agar medium containing 25 tannery wastewater after incubation time at 48 hours. The growth curve was also observed at a liquid medium containing tannery wastewater at 25 during 36 hours observation since at the 12th hour. Thus, Bacillus cereus LS2B could tolerate up to 25 of fresh untreated tannery wastewater in the solid and liquid medium.