The potential of Biogas Sludge Dairy Cattle with Fortification of Expired Milk Powder Waste and Eggshell as a Planting Media of White Oyster Mushroom (Pleurotus florida) on Mycelium Growth

This study aimed to determine the nutrient content of the growing media of sludge biogas waste from dairy cattle and expired milk waste and the growth of white oyster mushroom mycelium (Pleurotus florida). This study uses mixed media from dairy cattle biogas sludge waste and expired milk powder with different concentrations divided into 4 media treatments, 4 treatments then performed 3 repetitions to obtain 12 experimental units, the treatment is given code P0, P1, P2, P3 which has a media composition in the form of sawdust, biogas sludge, expired milk powder waste and eggshell, each treatment is distinguished in different concentrations of sludge and expired milk powder with different proportions namely P0 (0: 0), P1 (50: 50), P2 (25: 75) P3: (75: 25), P0 is used as a control because it does not use the two combination ingredients but uses bran as a source of nutrients and is a proportion commonly used by oyster mushroom farmers. Each media was then tested for its chemical content including water content, organic matter, crude fiber, C-organic, nitrogen, P-Total, and potassium. Then for the growing mycelium variable observed were the mycelium length, the percentage of growth of oyster mushroom mycelium, the first time to grow, and the time to fulfill mycelium. The data was processed using analysis of Variance-Completely Randomized Design Unidirectional and the average difference was tested using Duncan's Multiple Range Test (DMRT). Mushroom media nutrient content test results showed that the best results were in the planting media of sludge biogas waste from dairy cattle and expired milk waste in P3 treatment (75 dairy biogas sludge: 25 of expired milk waste) was able to increase nutrient in a c-organic variable, crude fiber, C / N ratio, and potassium are 19.61, 57.43, 3.179, and 0.887. The best results of mycelium growth in this study were the planting media of dairy cattle biogas sludge waste and expired milk waste in P2 treatment (25 of dairy cattle biogas sludge: 75 of expired milk waste) which was able to produce the best growth of the average variables of the daily mycelium length, percentage of growth mycelium, first time to grow and time to fulfill mycelium is 3.73 cm, 27.42, 4.33 days and 26.33 days. In this study, it can be concluded that the planting media from dairy cattle biogas sludge waste with the fortification of expired milk powder waste can be potentially used as an alternative media other than bran at the same time can increase the nutrient content in the mushroom media and stimulate the growth of mycelium better.