Rahayu, Indra Lesmana and Kusumawati, Asmarani (2021) Entrapment formulation for env-Tm gene based on chitosan low molecular weight as a jembrana disease virus vaccine candidate. In: International Conference on Bioenergy and Environmentally Sustainable Agriculture Technology (ICoN BEAT 2019).
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Abstract
Jembrana disease (JD) caused by Jembrana Disease Virus (JDV) becomes an obstacle in Bali cattle (Bos javanicus). The development of JD vaccines has a critical meaning to prevent losses in the B. javanicus in Indonesia and is one of the models in the development of the Human Immunodeficiency Virus (HIV) vaccine. The development of vaccines for JDV has carried out DNA vaccines that are expected to provide better immunological effects. This study aimed to determine the low molecular weight chitosan (LC) entrapment towards pEGFP-C1-env-Tm in the formation of Chitosan Nanoparticles Low/pEGFP-C1-env-Tm complex. The env-Tm gene was inserted in pEGFP-C1 into the pEGFPC1/ env-Tm construct transformed on the E. coli DH5α host. The construct was formulated into LC/pEGFP-C1/env-Tm complex with a low molecular weight chitosan concentration of 0.06 and the ratio of pEGFP-C1/env-Tm: LC (wt/wt) was 1:0.5-1:3. The complexes were then analyzed by gel retardation assay agarose 1 . The results of this study indicated that the best entrapment results of low molecular weight chitosan to pEGFPC1/ env-Tm was in the mass ratio of pEGFP-C1/env-Tm: LC was 1:2. The best formulation entrapment for env-Tm by low molecular weight chitosan 0.06 is in the rate 1:2. © The Authors, published by EDP Sciences, 2021.
Item Type: | Conference or Workshop Item (Paper) |
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Additional Information: | Cited by: 0; Conference name: 1st International Conference on Bioenergy and Environmentally Sustainable Agriculture Technology, ICoN BEAT 2019; Conference date: 7 November 2019 through 8 November 2019; Conference code: 166263; All Open Access, Gold Open Access |
Uncontrolled Keywords: | Agriculture; Chitosan; Escherichia coli; Genes; Molecular weight; Vaccines; Viruses; Chitosan nanoparticles; DNA vaccine; Gel-retardation assay; Human immunodeficiency virus; Immunological effects; Low molecular weight; Low molecular weight chitosans; Mass ratio; Diseases |
Subjects: | R Medicine > R Medicine (General) |
Divisions: | The Graduate School |
Depositing User: | Sri JUNANDI |
Date Deposited: | 22 Oct 2024 06:57 |
Last Modified: | 22 Oct 2024 06:57 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/5505 |