The Effect Of NaCl Salinity Stress To Phenolic Compound, Total Flavonoid And Antioxidant Activity Of Pegagan (Centella asiatica (L.) Urban) Leaves

Pegagan (Centella asiatica (L.) Urban) is a herbal plant that contain secondary metabolite compounds like phenol and flavonoid. NaCl salinity is one of abiotic stress that enhanced synthesis of some secondary metabolites in plants. This study was investigated the effect of NaCl salinity stress to phenolic compound, total flavonoid and antioxidant activity of pegagan leaves. Pegagan were treated with five different NaCl concentrations, 0 mM (1), 50 mM (2), 100 mM (3), 150 mM (4) and 200 mM (5) for a week. Morphological leaves were observed for the present of necrotic symptom. Phenolic compound and total flavonoid content were measured using spectrophotometer at wavelength 765 nm and 415 nm. Antioxidant activity was measured based on DPPH method. The result showed that increasing NaCl concentration cause increasing necrotic spot in leaves. Phenolic compound, total flavonoid and antioxidant activity are increased by increasing NaCl concentration. The result indicated that phenol and flavonoid have important role in plant defense mechanism against NaCl toxicity effects. © The Authors, published by EDP Sciences. This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/).