Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Putri, Wahyu Aristyaning and Rahayu, Hanum Mukti and Khasanah, Anis Uswatun and Sembiring, Langkah and Kawaichi, Masashi and Purwestri, Yekti Asih (2021) Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene. Indonesian Journal of Biotechnology, 26 (4). 206 – 213. ISSN 08538654

[thumbnail of 65989-240364-2-PB.pdf] Text
65989-240364-2-PB.pdf
Restricted to Registered users only

Download (738kB)

Abstract

Streptomyces is one of mercury-resistant bacteria which can convert Hg2+ into nontoxic Hg0. This study aimed to identify mercury-resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small-scale gold mining (ASGM) area and clone merA gene to the cloning and expression vectors. Molecular identification was conducted using 16s rRNA gene with the maximum likelihood algorithms. Results revealed that the AS1 and AS2 strains were a group of Streptomyces ardesiacus and the BR28 strain was closed to Brevibacillus agri. The AS2 merA gene was cloned to pMD20 cloning vectors, pGEX-5x-1 and pET-28c expression vectors. The transformation was successfully performed in BL21 and DH5α competent cells. The full length of the merA gene was confirmed to be 1,425 bp. This study is the first research on identifying mercury-resistant Streptomyces and cloning the full-length merA gene in Indonesia. © 2021 Universitas Gadjah Mada, Research Center for Biotechnology. All rights reserved.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access
Subjects: Biology
Divisions: Faculty of Biology > Master Program in Biology
Depositing User: Sri JUNANDI
Date Deposited: 28 Oct 2024 03:14
Last Modified: 28 Oct 2024 03:14
URI: https://ir.lib.ugm.ac.id/id/eprint/5639

Actions (login required)

View Item
View Item