Re-epithelization and density of collagen fibers on wound healing of mice's skin (Mus musculus) that treated with combination of chitosan membrane and eel (Monopterus albus) mucous

Untreated skin wound can inhibit the wound healing process. Chitosan and eel mucous have biodegradable, biocompatible, and antimicrobial properties. This study aimed to determined the potential of combination between chitosan membrane and eel mucous extract in skin wound re-epithelization and collagen fibers deposition. Thirty adult male mice were divided into 5 groups, 6 animals/group. Skin wound were perform by punch biopsy in 0.5 cm diameter. Group I-IV respectively recieved: 10 povidone iodine; eel mucus extract and gauze; combination of 1 chitosan membrane and eel mucus; 1 chitosan membrane. Group V without therapy. Therapy was given once a day for 10 days. At the 5th and 10th half of animals/group were euthanized. Wound skin area were collected for hematoxylin-eosin and Mallory-azan stainings. Epithelial thickness and collagen density were observed and analyzed statistically. The results showed significant difference in epithelial thickness on the 5th and 10th days (P ≤ 0.05) in combination group. Percentage of collagen density at 10th day of combination group showed significantly difference (P ≤ 0.05) compared with control. The conclusion is combination of 1 chitosan membrane and eel mucous extract has the most potential to re-epithelization and increase density of collagen fibers in skin wound healing. © The Authors, published by EDP Sciences.