Isolation, Identification, and Antimicrobial Susceptibility Test of Bacteria from Vulva Swab of African Pygmy Hedgehog (Atelerix albiventris) and Sunda Porcupine (Hystrix javanica)

Atelerix albiventris and Hystrix javanica are currently traded as pets or consumed in Indonesia, but there has been no research about bacteria from the vulva swab before. This research aims to isolate and identify bacteria from the vulva swabs of Atelerix albiventris and Hystrix javanica, and identify their antibiotic susceptibility. Samples were isolated by blood agar plates and selective media and identified by biochemical tests. Kirby Bauer's disk diffusion method was used for the antimicrobial susceptibility test. The result showed that from Atelerix albiventris was isolated and identified Escherichia coli and Proteus mirabilis, while from Hystrix javanica was isolated and identified Escherichia coli. The identified Escherichia coli was sensitive to Amikacin, Amoxycillin, Ampicillin, Enrofloxacin, Fosfomycin, Chloramphenicol, Tetracycline, Trimethoprim, and Kanamycin; intermediate to Streptomycin; and resistant to Erythromycin and Penicillin G. The identified Proteus mirabilis was sensitive to Amikacin, Amoxycillin, Kanamycin, Enrofloxacin, and Fosfomycin; and resistant to Erythromycin, Penicillin G, Streptomycin, Ampicillin, Chloramphenicol, Tetracycline, and Trimethoprim. This research concludes that Escherichia coli and Proteus mirabilis were isolated from vulva swab of Atelerix albiventris and Hystrix javanica, Escherichia coli was sensitive to eight antibiotics, while Proteus mirabilis was sensitive to five antibiotics. © The Authors, published by EDP Sciences.