EVALUATION OF Lactobacillus plantarum AND Lactococcus lactis ISOLATED FROM DUCK EXCRETA AS POTENTIAL PROBIOTICS FOR CHICKEN NUTRITION

Since the antibiotic growth promoters (AGP) were banned, probiotics have become one of the increasingly relevant products to be developed in the poultry industry. Thereafter lactic acid bacteria (LAB) isolated from excreta have the potential as probiotics. The aim of this study was to determine the potential probiotic properties of Lactobacillus and Lactococcus isolated from duck excreta. The experiment was conducted in the following two processes, in vitro and in vivo. Based on identification with API 50CHL and 16s rRNA, LAB isolated confirmed Lactobacillus plantarum (L. plantarum) and Lactococcus lactis (Lc. lactis). The strains showed tolerance to variation of pH 2.0 to 5.0, and level bile salt 0.05 to 0.30; the ability of intestinal adhesion and antibacterial activity against Escherichia coli. The strain was safe to use as a probiotic because is negative for hemolytic activity and high sensitivity to antibiotics. In a In vivo study, a total of 100 Lohmann broilers (7-day-old) were divided into 4 treatment groups included of control (P0), L. plantarum BJ3 107 cfu/bird (P1), Lc. lactis K5 107 cfu/bird (P2), and L. plantarum BJ3 + Lc. lactis K5 107 cfu/bird (P3) and received probiotic as orally. The result showed probiotic supplementation affected growth performance of broilers. Probiotic supplementation reduced feed intake (FI) during experimental period (p<0.031), but the feed conversion ratio (FCR) and body weight gain (BWG) were not affected. The P2 group showed the lowest FI. In conclusion, L. plantarum BJ3 and Lc. lactis K5 isolated from duck excreta can be considered as probiotics for chicken nutrition. © 2021. All Rights Reserved.