High prevalence of circulating DS-1-like human rotavirus A and genotype diversity in children with acute gastroenteritis in Thailand from 2016 to 2019

Pasittungkul, Siripat and Lestari, Fajar Budi and Puenpa, Jiratchaya and Chuchaona, Watchaporn and Posuwan, Nawarat and Chansaenroj, Jira and Mauleekoonphairoj, John and Sudhinaraset, Natthinee and Wanlapakorn, Nasamon and Poovorawan, Yong (2021) High prevalence of circulating DS-1-like human rotavirus A and genotype diversity in children with acute gastroenteritis in Thailand from 2016 to 2019. PeerJ, 9. ISSN 21678359

[thumbnail of Siripat_SV.pdf] Text
Siripat_SV.pdf - Published Version
Restricted to Registered users only

Download (3MB) | Request a copy

Abstract

Background: Human rotavirus A (RVA) infection is the primary cause of acute gastroenteritis (AGE) in infants and young children worldwide, especially in children under 5 years of age and is a major public health problem causing severe diarrhea in children in Thailand. This study aimed to investigate the prevalence, genotype diversity, and molecular characterization of rotavirus infection circulating in children under 15 years of age diagnosed with AGE in Thailand from January 2016 to December 2019. Methods: A total of 2,001 stool samples were collected from children with gastroenteritis (neonates to children <15 years of age) and tested for RVA by real-time polymerase chain reaction (RT-PCR). Amplified products were sequenced and submitted to an online genotyping tool for analysis. Results: Overall, 301 (15.0) stool samples were positive for RVA. RVA occurred most frequently among children aged 0-24 months. The seasonal incidence of rotavirus infection occurred typically in Thailand during the winter months (December-March). The G3P8 genotype was identified as the most prevalent genotype (33.2%, 100/301), followed by G8P8 (10.6%, 32/301), G9P8 (6.3%, 19/301), G2P4 (6.0%, 18/301), and G1P6 (5.3%, 16/301). Uncommon G and P combinations such as G9P4, G2P8, G3P4 and G3P9 were also detected at low frequencies. In terms of genetic backbone, the unusual DS-1-like G3P8 was the most frequently detected (28.2%, 85/301), and the phylogenetic analysis demonstrated high nucleotide identity with unusual DS-1-like G3P8 detected in Thailand and several countries. Conclusions: A genetic association between RVA isolates from Thailand and other countries ought to be investigated given the local and global dissemination of rotavirus as it is crucial for controlling viral gastroenteritis, and implications for the national vaccination programs. © 2021 Pasittungkul et al.

Item Type: Article
Additional Information: Cited by: 7; All Open Access, Gold Open Access, Green Open Access
Uncontrolled Keywords: adolescent; bootstrapping; child; DNA sequencing; feces; genotype; human; Human rotavirus; incidence; maximum likelihood method; molecular diagnosis; nucleotide sequence; phylogeny; prevalence; real time polymerase chain reaction; Review; Rotavirus infection; Thailand; viral gastroenteritis; virus genome
Subjects: S Agriculture > SF Animal culture
Divisions: Vocational School
Depositing User: Sri JUNANDI
Date Deposited: 03 Sep 2024 04:30
Last Modified: 03 Sep 2024 04:30
URI: https://ir.lib.ugm.ac.id/id/eprint/5827

Actions (login required)

View Item
View Item