Wahyuningsih, Rina and Rusman, Rusman and Nurliyani, Nurliyani and Rohman, Abdul and Erwanto, Yuny (2021) Potency of pepsin soluble collagen from indonesian local goat skin as an antioxidant. American Journal of Animal and Veterinary Sciences, 16 (3). 144 – 151. ISSN 15574555
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Abstract
Pepsin Soluble Collagen (PSC) hydrolyses is derived from the collagen extraction process from Indonesia local goat skin (Kacang) was carried out in some previous studies. This study is considering the first research used the collagen from the goat skin as an antioxidant compound. The main purpose of study were to investigate antioxidant activity of PSC hydrolysis after pepsin treatment. The experiment was applied enzymatic treatment using pepsin as 0.1 U unit/g per g collagen for various (0, 30, 60, 90 and 120 min) hydrolysis time under 37°C to determine the degree of hydrolysis. Collagen solubility, protein molecular weight and value of radical scavenging activities were observed. The result of the study showed that the highest of degree of hydrolysis of PSC from goat skin found at 90 min incubation (20.05±0.76). The highest collagen solubility of PSC was hydrolyzed for 30 min with the value 2.59 mg/mL. PSC before hydrolysis has molecular weight of 57.82-162.06 kDa and after hydrolysis using pepsin and incubation at 37ºC for 120 min, the molecular weight decreased into 9.09-46.75 kDa. The concentration of 500 ppm of PSC after hydrolysis for 90 min has 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging activity 59.35 with IC50 198.59 ppm. This study indicated that hydrolysis time influenced solubility, molecular weight and radical scavenging activity of PSC hydrolysate from Indonesia local goat skin. © 2021 Rina Wahyuningsih, Rusman, Nurliyani, Abdul Rohman and Yuny Erwanto.
Item Type: | Article |
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Additional Information: | Cited by: 3; All Open Access, Gold Open Access |
Uncontrolled Keywords: | Antioxidant Properties, Molecular Weight, Pepsin Soluble Collagen, Protein Hydrolysates |
Subjects: | S Agriculture > SF Animal culture |
Divisions: | Faculty of Animal Sciences > Department of Animal Products Technology |
Depositing User: | Sri JUNANDI |
Date Deposited: | 02 Sep 2024 07:24 |
Last Modified: | 02 Sep 2024 07:24 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/5900 |