The potency of sansevieria trifasciata and s. Cylindrica leaves extracts as an antibacterial against pseudomonas aeruginosa

Dewatisari, Whika Febria and Nugroho, Laurentius Hartanto and Retnaningrum, Endah and Purwestri, Yekti Asih (2021) The potency of sansevieria trifasciata and s. Cylindrica leaves extracts as an antibacterial against pseudomonas aeruginosa. Biodiversitas, 22 (1). 408 – 415. ISSN 1412033X

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Abstract

Sansevieria trifasciata and Sansevieria cylindrica are the major herbs in Indonesia, which contain several bioactive compounds as potential sources of antibacterial agents. This study aims to evaluate the antibacterial activity of S. trifasciata and S. cylindrica leaves extract and fraction against Pseudomonas aeruginosa and to identify its bioactive compounds. Crude ethanolic extract of S. trifasciata and S. cylindrica leaves were tested for their antimicrobial activity by disk diffusion method against P. aeruginosa. S. trifasciata showed strong antibacterial activity with an inhibition zone of 18.3 mm compared to S. cylindrica. Different concentrations of extract i.e. 4 mg/mL, 8 mg/mL, 16 mg/mL, 32 mg/mL, 64 mg/mL, 128 mg/mL and 256 mg/mL were tested for their minimum inhibitory concentrations (MIC). The MIC results showed that the S. trifasciata extract was able to inhibit bacterial growth at a concentration of 32 mg/mL. Results of vacuum liquid chromatography (VLC) and thin-layer chromatography (TLC) revealed that only fraction 3 showed the highest antibacterial activity at 16 mg/mL. In TLC bioautography analysis, fraction 3 showed a clear zone at Rf 0.93. The phytochemical analysis showed that terpenoid, phenolic, triterpenoid, and flavonoid compounds were found in S. trifasciata extract that were associated with antibacterial activity.

Item Type: Article
Additional Information: Library Dosen
Uncontrolled Keywords: Antibacterial, ethanolic extract, Pseudomonas aeruginosa, Sansevieria cylindrica, Sansevieria trifasciata
Subjects: Biology
Divisions: Faculty of Biology > Undergratuate Program in Biology
Depositing User: Sri JUNANDI
Date Deposited: 29 Oct 2024 03:55
Last Modified: 29 Oct 2024 03:55
URI: https://ir.lib.ugm.ac.id/id/eprint/5937

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