Clonal propagation of rare orchid species Paphiopedilum spp. (Orchidaceae) to save Indonesian biodiversity

Paphiopedilum spp. is one of the terrestrial orchids that is interesting as an ornamental plant. This orchid
flower has a distinctive characteristic, namely its labellum resembles a sac, so it is sought after by ornamental
flower collectors because of its uniqueness. This orchid is included in the Appendix I group according to
CITES, namely a critical species that is threatened with extinction, so it needs to be conserved both in situ
and ex situ. Ex situ conservation can be done by propagating orchids using in vitro culture techniques. The
objective of this research is to carry out mass propagation of 3 species of Paphiopedilum spp orchids: P. supardii, P. primulinum, P. glaucophyllum through somatic embryogenesis and to characterize the existence of
embryo gene RKD4 homolog in Paphiopedilum orchids. The method is to culture the nodes slices in dark conditions for 2 weeks, and then changed to photoperiod mode 16:8 hours (dark:light) on 1
/2 MS media plus a
combination of 2,4-D or NAA with TDZ. The somatic embryo were analyzed anatomically. This method is an
improvement over previously reported methods for Paphiopedilum spp. due to its efficiency and reproducibility for conservation. We concluded that 1
/2 MS media with a combination of NAA or 2,4D with TDZ were
able to induce somatic embryogenesis. The result show that 3 mg L�1 NAA was the best PGRs for PLBs induction in P. supardii (50 %), 1 mg L�1 2,4-D + 0.5 mg L�1 TDZ was the best PGRs for callus embryogenic induction
in P. primulinum (20 %), and 1 mg L�1 NAA was the best PGRs for PLBs induction in P. glaucophyllum (33 %).
© 2024 SAAB. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI
training, and similar technologies.