Polymorphism analysis of the Insulin-like Growth Factor-1 (IGF-1) gene encoding growth in F5 Golden Kamper chicken (Gallus gallus domesticus)

Golden Kamper Chicken is a chicken cross between pelung and
layer chickens with good meat and egg productivity. Insulin-like growth
factor-1 (IGF-1) gene is one factor affecting the speed of chicken growth.
This research aims to study the character of F5 Golden Kamper chickens and
analyze the IGF-1 gene to determine its effect on chicken growth. The
research will be done by hybridizing the F4 Golden Kamper hen and rooster,
raising brood stock and DOC, egg collection, growth data collection, blood
collection, DNA isolation, DNA amplification, electrophoresis, and
sequencing. The observed parameters are DOC weight for the first seven
weeks and polymorphism of the IGF-1 gene. Data was analyzed using
Microsoft Excel, Genestudio, MEGA11, and IBM SPSS 25 software.
Sequencing data were processed using the Genestudio application,
alignment with MEGA11, and genotype-haplotype associations using
Pearson correlation and Linear Regression F test. The results showed that
the molecular marker polymorphism of the IGF-1 gene was found at 12
mutation points. IGF-1 gene polymorphism did not correlate with the weight
of F5 Golden Kamper chickens.