Optimization of Medium for Lipid Production from Lipomyces maratuensis InaCC Y720 Using Statistical Experiment Design

Audinah, Liya and Kanti, Atit and Ilmi, Miftahul (2024) Optimization of Medium for Lipid Production from Lipomyces maratuensis InaCC Y720 Using Statistical Experiment Design. Pertanika Journal of Tropical Agricultural Science, 47 (3): 10. pp. 905-921. ISSN 15113701

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Abstract

Lipomyces maratuensis InaCC Y720 is a potential novel oleaginous yeast. Media-based production optimization has never been carried out using this strain. This study aims to define an optimized medium from 12 medium component factors, where the Taguchi method is used for screening significant factors of medium and the response surface methodology (RSM) is used to optimize the concentration of significant factors. According to Taguchi, glucose, yeast extract, and magnesium sulfate (MgSO4) have a significant influence on lipid accumulation, with their concentrations maintained at optimal levels through RSM optimization. Conversely, potassium dihydrogen phosphate, sodium hydrogen phosphate, and calcium chloride inhibit lipid accumulation, and copper(II) sulfate has the least influence, categorizing them as eliminated factors. The RSM-optimized medium increased lipid content by 3.6-fold compared to the initial medium. Glucose and yeast extract showed a positive correlation with lipid accumulation, suggesting poten

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access
Uncontrolled Keywords: Initial media, nutrients, oleaginous yeast, optimized media, response surface methodology, Taguchi method
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Biology > Doctoral Program in Biology
Depositing User: Rusna Nur Aini Aini
Date Deposited: 09 Oct 2024 08:14
Last Modified: 09 Oct 2024 08:14
URI: https://ir.lib.ugm.ac.id/id/eprint/6511

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