Clonal propagation of rare orchid species Paphiopedilum spp. (Orchidaceae) to save Indonesian biodiversity

Istiqomah, Nurul and Indriani, Hesti and Wijaya, Yusril Ihza Farhan and Safitri, Safitri and Yalapuspita, Dyah Carinae and Handini, Elizabeth and Diantina, Surya and Aprilianti, Popi and Semiarti, Endang (2024) Clonal propagation of rare orchid species Paphiopedilum spp. (Orchidaceae) to save Indonesian biodiversity. South African Journal of Botany, 172 (1): 10. 779 -785. ISSN 02546299

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Abstract

Paphiopedilum spp. is one of the terrestrial orchids that is interesting as an ornamental plant. This orchid flower has a distinctive characteristic, namely its labellum resembles a sac, so it is sought after by ornamental flower collectors because of its uniqueness. This orchid is included in the Appendix I group according to CITES, namely a critical species that is threatened with extinction, so it needs to be conserved both in situ and ex situ. Ex situ conservation can be done by propagating orchids using in vitro culture techniques. The objective of this research is to carry out mass propagation of 3 species of Paphiopedilum spp orchids: P. supardii, P. primulinum, P. glaucophyllum through somatic embryogenesis and to characterize the existence of embryo gene RKD4 homolog in Paphiopedilum orchids. The method is to culture the nodes slices in dark conditions for 2 weeks, and then changed to photoperiod mode 16:8 hours (dark:light) on ½ MS media plus a combination of 2,4-D or NAA with TDZ. The somatic embryo were analyzed anatomically. This method is an improvement over previously reported methods for Paphiopedilum spp. due to its efficiency and reproducibility for conservation. We concluded that ½ MS media with a combination of NAA or 2,4D with TDZ were able to induce somatic embryogenesis. The result show that 3 mg L−1 NAA was the best PGRs for PLBs induction in P. supardii (50 ), 1 mg L−1 2,4-D + 0.5 mg L−1 TDZ was the best PGRs for callus embryogenic induction in P. primulinum (20 ), and 1 mg L−1 NAA was the best PGRs for PLBs induction in P. glaucophyllum (33 ). © 2024 SAAB

Item Type: Article
Additional Information: Cited by: 0
Uncontrolled Keywords: Micropopagation Paphiopedilum spp. Somatic embryogenesis Plant growth regulators RKD
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Biology > Doctoral Program in Biology
Depositing User: Rusna Nur Aini Aini
Date Deposited: 23 Sep 2024 07:32
Last Modified: 23 Sep 2024 07:32
URI: https://ir.lib.ugm.ac.id/id/eprint/6588

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