Palupi, Endah Sri and Retnoaji, Bambang and Astuti, Pudji and Alamsyah, Firman and Taruno, Warsito Purwo and Pratiwi, Rarastoeti (2024) Alternating Current-Electric Field Inducing Chorio Allantoic Membrane (CAM) Angiogenesis through Exogenous Growth Factor Intervention. Current Applied Science and Technology, 24 (4): 1. pp. 1-10. ISSN 25869396
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Abstract
Angiogenesis is widely used in various therapies by promoting or inhibiting the formation of new blood vessels. The use of Alternating Current-Electric Fields (AC-EF) in Electro-Capacitive Cancer Therapy (ECCT) showed its potential as an anti-cancer device, and is characterized by its anti-proliferative and pro-apoptotic effects. However, the role of AC-EF in angiogenesis remains unclear. To investigate the effects of AC-EF on CAM angiogenesis, we used the ex ovo culture method of chorioallantoic membrane (CAM). A basic fibroblast growth factor (bFGF) dose of 30 ng/µL was administered as an exogenous growth factor. The ECCT device, generating AC-EF of 150 kHz and 18 Vpp, was exposed to the CAMs. Subsequently, the 24 CAMs of chick embryo were divided into four groups. Two groups were non-bFGF-induced CAM, while the other two were bFGF-induced CAM, and each group was exposed either with or without AC-EF. The vascularization was evaluated through macroscopic observation, while vascular endothelial growth factor A (VEGFA) gene expression was measured using qPCR. The data were statistically analyzed using ANOVA with GraphPad Prism 9.5. The results showed that an AC-EF exposure had no effects on normal CAM angiogenesis (P>0.05). Moreover, VEGFA gene expression did not show significant upregulation (P>0.05) in the bFGF-induced CAM with or without AC-EF exposure. Interestingly, the number of new blood vessels was significantly higher (P<0.05) in the bFGF-induced with AC-EF exposure than in the non-bFGF-induced group. In conclusion, AC-EF of ECCT did not affect normal angiogenesis. AC-EF may trigger CAM angiogenesis with bFGF induction. This observation suggested that AC-EF of intermediate frequency could enhance angiogenesis by administration of external growth factors, offering a potential avenue for addressing obstructive vascular conditions. © 2024, King Mongkut's Institute of Technology Ladkrabang. All rights reserved.
Item Type: | Article |
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Additional Information: | Cited by: 0; All Open Access, Hybrid Gold Open Access |
Uncontrolled Keywords: | AC-EF;angiogenesis;bFGF;CAM;vascularization;VEGFA |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Biology > Doctoral Program in Biology |
Depositing User: | Rusna Nur Aini Aini |
Date Deposited: | 13 Sep 2024 04:03 |
Last Modified: | 13 Sep 2024 04:03 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/6651 |