Molecular Identification of ABC2 Transporter Gene Encode Protein Ngawi Trypanosoma evansi Isolate that suspected resistance to Isometamidium Chloride

Wusahaningtyas, Lu’lu’ Sahara and Nuryady, Moh Mirza and Firdausy, Lintang Winantya and Zs, Ahmad Fahrurrozi and Nurcahyo, R. Wisnu (2021) Molecular Identification of ABC2 Transporter Gene Encode Protein Ngawi Trypanosoma evansi Isolate that suspected resistance to Isometamidium Chloride. BioMIC, 41: 06003. pp. 1-6. ISSN 22731709

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Abstract

This study aims to determine the profile of the ABC2 encoding transporter on Trypanosoma evansi (T. evansi) Ngawi isolates, Indonesia, exposed with Isometamidium Chloride (ISM). This study used blood samples of mice containing Trypanosoma evansi that had been exposed with ISM 0.05 mg/kg BW, ISM 0.1 mg/kg BW and ISM 0.3 mg/kg BW for 4 weeks, and control group. Blood samples were extracted and amplified using primers. ABC2 F 5 'GCTTGTCCGACCATCTTGCA 3' and ABC2 R 5 'AGGTCCACTCCCATGCTACA 3' that produced 350 basepairs (bp). The sequencing results were then analyzed using BLAST and MEGA 7.0. There was 1 deference nucleotide (107) derived from multiple alignments, while in amino acids there was no difference in all samples. Trypanosoma evansi which was
exposed with ISM does not have many differences in nucleotide or amino acid and only one type of mutation.
The ABC2 Transporters of four groups of T.evansi have high similarity to ABC Transporters of T. brucei
gambiense, T. brucei brucei, and T. brucei brucei (Tbabc2). Therefore, further research on the ABC2 Transporter gene is needed.

Item Type: Article
Uncontrolled Keywords: ABC2 Transporter Gene, Trypanosoma evansi, Isometamidium Chloride, resistance.
Subjects: S Agriculture > SF Animal culture
Divisions: Faculty of Veterinary Medicine
Depositing User: Erlita Cahyaningtyas Cahyaningtyas
Date Deposited: 18 Sep 2024 03:06
Last Modified: 18 Sep 2024 03:06
URI: https://ir.lib.ugm.ac.id/id/eprint/7121

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