Unsunnidhal, Lalu and Jannah, Raudatul and Haris, Abdul and Supinganto, Agus and Kusumawati, Asmarani (2021) Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-SB3- HBcAg. BioMIC 2021, 41: 07003. pp. 1-6. ISSN 22731709
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Abstract
Hepatitis B virus (HBV) is a DNA virus that causes hepatitis in humans. This study aims to prepare a Hepatitis DNA vaccine. he optimized base sequence of the SB3-HBcAg gene was derived from the nucleotide base sequence of the Hepatitis B core antigen B3 HBcAg subgenotype, and then Cloning of the pcDNA3.1-SB3-+%F$J KDV EHHQ VXFFHVVIXOO\ SHUIRUPHG RQ (� FROL '+�Į DQG FRQILUPHG E\ 3&5�
restriction analysis and sequencing. The propagated plasmids were prepared as DNA-chitosan complex and
physiochemical characterized using Particle Size Analyzer. Complex with a 4:1 (wt/wt) ratio of DNA with 0.04% concentration and chitosan have a mean diameter of 231.7 nm and zeta potential +12.3 mV and the value of Cytotoxicity Assay 80-90% as compared to the untreated cells that used as negative control, so it can be concluded that nanoparticles chitosan has good potential as a carrier agent for pcDNA3.1-SB3-
HBcAg.
Item Type: | Article |
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Uncontrolled Keywords: | A codon optimization, Chitosan nanoparticles, HBcAg, Hepatitis B virus, Delivery System |
Subjects: | S Agriculture > SF Animal culture |
Divisions: | Faculty of Veterinary Medicine |
Depositing User: | Erlita Cahyaningtyas Cahyaningtyas |
Date Deposited: | 18 Sep 2024 07:18 |
Last Modified: | 18 Sep 2024 07:18 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/7309 |