Chalcone-3 Inhibits the Proliferation of Human Breast Cancer MDA-MB-231 Cell Line

Padauleng, Novrita and Mustofa, Mustofa and Wahyuningsih, Tutik Dwi and Purnomosari, Dewajani (2023) Chalcone-3 Inhibits the Proliferation of Human Breast Cancer MDA-MB-231 Cell Line. Asian Pacific Journal of Cancer Prevention, 24 (2). pp. 683-691. ISSN 15137368

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Abstract

Objective: Chalcone-3 has been shown to be cytotoxic and selective against luminal subtype breast cancer cell lines, which are suspected to occur through the mechanism of epidermal growth factor receptors (EGFR) inhibition. However, the cytotoxic effect has never been tested on cell strains from patients with triple negative breast cancer (TNBC), where EGFR expression is known to increase. This study aimed to identify the role of chalcone-3 in one of the downstream targets of EGFR as an antiproliferative agent. Methods: Chalcone-3 was examined for its effect on proliferation in human breast cancer MDA-MB-231 cell lines. The percentage of proliferation inhibition was analyzed using methyl-thiazol tetrazolium assay. Flow cytometry was used to analyze the population of cell cycle distribution and the expression of cyclin-D1 and pEGFR. Results: Chalcone-3 inhibited the proliferation of MDA-MB-231 cells in a dose and time-dependent manner with an IC50 value of 17.98±6.36 μg/mL by inducing cell cycle arrest at the G2/M phase. Flow cytometry assays showed that chalcone-3 significantly reduced the expression of pEGFR and cyclin-D1, contributing to cell cycle arrest. Conclusion: Chalcone-3 might have potential as an anti-proliferative drug to treat TNBC. © 2023,Asian Pacific Journal of Cancer Prevention.All Rights Reserved.

Item Type: Article
Uncontrolled Keywords: anti-proliferative drug; Chalcone-3; MDA-MB-231; triple negative breast cancer
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Mathematics and Natural Sciences > Chemistry Department
Depositing User: Ismu WIDARTO
Date Deposited: 20 Sep 2024 06:31
Last Modified: 20 Sep 2024 06:31
URI: https://ir.lib.ugm.ac.id/id/eprint/7375

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