The effect of supplementation of Isomaltooligosaccharide and emulsified medium-chain triglycerides on blood glucose, b-hydroxybutyrate and calorie intake in male Wistar rats

Background: A high concentration of energy substrate in circulation will suppress appetite and subsequent food intake. Mediumchain triglycerides (MCT) is a natural exogenous ketone source that can act as an energy substrate, increase blood ketone and suppress appetite. MCT emulsification increases its ketogenic properties. Interestingly, Isomaltooligosaccharide (IMO), a form of
dietary fiber, can absorb and retain water, induce the stomach wall distention, and therefore could induce the afferent signal that causes appetite suppression. Despite those facts, there are no data available on whether a food supplement contains a combination of IMO and emulsified MCT (FC-MCT) could induce more appetite suppression.

Objectives: This research investigated the effect of FC-MCT on blood glucose, b-hydroxybutyrate (bHB), and calorie intake in male Wistar rats.

Methods: A total of 31 male Wistar rats were divided into four groups. 1) The MC group (n ¼ 8) - MCT 10 ml/kg of body weight; 2) the FC group (n ¼ 8) - combination of IMO and emulsified coconut oil or FC-CO 100% (w/v) 15 ml/kg of body weight; 3) the FM group (n ¼ 7) - FC-MCT 100% (w/v) 15 ml/kg of body weight; and 4) the SU group (n ¼ 8) - sucrose solution 20% (w/v) 15 ml/kg of body