Antimicrobial sensitivity of Avibacterium paragallinarum isolates from layers in the special region of Yogyakarta, Indonesia

Background and Aim: Infectious coryza (IC) is an upper respiratory disease of chicken caused by Avibacterium
paragallinarum. Its clinical symptoms are swollen face and malodorous sinus exudate. This study was conducted to
determine the antimicrobial sensitivity of A. paragallinarum isolates from layers in the Special Region of Yogyakarta,
Indonesia.

Materials and Methods: The samples used in this study were 30 layers that showed IC symptoms. The colony and cell
morphology were observed with Gram staining; then, biochemical tests (catalase, oxidase, urease, indole, and motility tests, and carbohydrate fermentation tests using lactose, maltose, mannitol, and sorbitol) were performed to the suspected colony to identify A. paragallinarum. An antibiotic sensitivity test was performed using several antibiotic disks against A. paragallinarum isolates that were cultured on Mueller-Hinton Agar.

Results: Out of 30 samples, 24 samples (80%) were found positive for A. paragallinarum. All isolates were sensitive to ampicillin (AMP) and amoxicillin (AML) (100%), and chloramphenicol (C) (91.6%). The antibiotics with intermediate sensitivity were enrofloxacin (79.2%), fosfomycin (75%), and ciprofloxacin (54.2%). The isolates were most resistant to erythromycin (100%), followed by tetracycline (87.5%), streptomycin (83.3%), doxycycline and kanamycin (70.8%), and trimethoprim (62.5%).

Conclusion: Out of the total samples, 24 samples (80%) from layers with IC symptoms were identified biochemically as
A. paragallinarum. It was sensitive to AMP, AML, and C.