Widayanti, Rini and Kusumaastuti, Ken Ayik and Novi, Joana Martha and Adani, Fadila Khairuna and Gultom, Catrine Relia Patrecia and Prastiti, Ayuning Devina and Nugroho, Herjuno Ari and Pakpahan, Suhendra (2021) Genetic variation and phylogenetic analysis of Indonesian indigenous catfish (baung fish) based on mitochondrial 12S rRNA gene. Veterinary World, 14 (3). pp. 751-757. ISSN 09728988
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Abstract
Background and Aim: Baung fish is an essential commodity in Indonesia; however, few studies have explored the genetic
diversity of Indonesian catfish. Thus, this study aimed to analyze the genetic variation and phylogenetic relationships among Indonesian catfish based on the mitochondrial 12S ribosomal RNA (rRNA) gene.
Materials and Methods: In total, 28 catfish were collected from nine rivers in seven provinces and from the Indian
Ocean. Catfish genomes were obtained from epaxial and hepaxial muscle samples. The mitochondrial 12S rRNA gene was
amplified by polymerase chain reaction using a pair of primers (Baung12SF and Baung12SR). The 12S rRNA sequences
were analyzed using MEGA X to determine genetic variation and phylogenetic relationships.
Results: In total, 178 variation sites in the 12S rRNA gene were substituted among Indonesian catfish. The genetic distance between all Indonesian catfish samples was 0.1-16.0%. The closest genetic distance was between MP and PM catfish, whereas the farthest genetic distances were between BF and EM and PD and EM. For the entire population, based on mean diversity calculations, the number of base substitutions per site was 0.08.
Conclusion: Indonesian catfish were divided into four clades based on the 12S rRNA gene. The catfish MP, KR, PM, MS,
BB, and KS were grouped with Hemibagrus nemurus, the catfish EM was grouped with Mystus vittatus, the catfish BSBJ
was grouped with Pangasius pangasius, and the catfish PD and BF were grouped with Netuma thalassina.
Item Type: | Article |
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Uncontrolled Keywords: | 12S ribosomal RNA gene, baung fish, Hemibagrus nemurus, Indonesian catfish, phylogenetic |
Subjects: | S Agriculture > SF Animal culture |
Divisions: | Faculty of Veterinary Medicine |
Depositing User: | Erlita Cahyaningtyas Cahyaningtyas |
Date Deposited: | 26 Sep 2024 07:30 |
Last Modified: | 26 Sep 2024 07:30 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/7572 |