Effect of Duwet fruit (Syzgium cumini) extract on MDA level and Caspase 3 expression in Rat (Rattus sp) Testes exposed to cigarette smoke

This research evaluates the MDA levels, number and apoptosis of spermatogenic cells in Rat provided with duwet fruit extract and exposed to cigarette smoke towards. The study
involved 4 experimental animal groups and 6 replications, where K0 was the negative control group, K1 was exposed the cigarette smoke, K2 was given orally duwet fruit, and K3 was given orally duwet fruit after being exposed to cigarette smoke. Therefore, data obtained were analyzed using One way Anova test. The results showed significantly lower number of
spermatocytes and MDA levels in the testes in all groups compared to K1 at p <0.05. Spermatogenic cell apoptosis occurred in all groups, and there was also a high tendency for reduction in K2 compared to K1, although not significant at p > 0.05. In addition, apoptosis was expressed by Caspase 3 in the testes. The highest MDA levels found in K1. This
phenomenon was assumed to have resulted in lower numbers of spermatocytes. These decreased number of spermatocytes were related with the decline in number of spermatogenic
cells prone to apoptosis. In conclusion, duwet fruit had the ability to reduce free radicals with the tendency to inhibit spermatogenesis. This case features an increase in spermatocytes and a potential decline in the incidence of spermatogenic cell apoptosis.