Effect of 10 kepok banana peel extract gel (musa paradisiaca linn. kepok) on periodontal regeneration process of Wistar rat

Objective: The aim of our study was to determine the effect of application of 10 Kepok banana peel extract gel (Musa paradisiaca linn. Kepok) on the periodontal regeneration process in periodontal healing of Wistar rat (Rattus norvegicus). Material and Methods: Fourty-eight Wistar rats were divided into 3 groups, namely 16 rats as the positive control group (Aloclair gel), 16 rats as the negative control group (CMC-Na gel 2) and 16 rats as the treatment group (10 Kepok banana peel extract gel). The induction of periodontitis was performed using a modified ligation technique for 7 days with an additional injection of Aggregatibacter actinomycetem-comitans bacteria on the first day. Wistar rats were euthanized on days 1, 3, 5, 7, and 14 for histological analysis to evaluate the number of macrophages, lymphocytes, osteoclasts, osteoblasts and fibroblasts. Radiographs were taken on day 21 using digital periapical radiograph (PaloDex Oy). Data were analyzed using Two-Way ANOVA and Post Hoc LSD tests. Results: Data analysis showed a significant difference in the cell numbers among the groups. A decreasing number of lymphocytes, macrophages, and osteoclasts could already be observed since the 1st day. The fibroblasts and osteoblasts of the treatment group already reached their peak on the 5th day, faster than the negative control and positive control groups that reached their peaks on the 7th day. Conclusion: The application of 10 Kepok banana peel extract gel (Musa paradisiaca linn. Kepok) caused a significant acceleration of the periodontal regeneration process in the periodontal healing of Wistar rat (Rattus norvegicus). © 2023 JDMFS. Published by Faculty of Dentistry, Hasanuddin University. All rights reserved.