The influence of platelet concentrates with and without metronidazole incorporation pre-centrifuge towards periodontal ligament broblast proliferation

Objective: Horizontal centrifugation was believed may improve platelet, leukocyte, and growth factors in the bu y coat. Previous study of platelet concentrates metronidazole incorporation was able to greatly inhibit bacteria. Metronidazole might help the proliferation with its ability to inhibit pro-in ammatory cytokines. This study intended to examine the in uence of C-PRF and i-PRF with and without metronidazole incorporation horizontal pre-centrifugation towards periodontal ligament broblast proliferation. Material and Methods: 2x10 broblast cells on the 96 well plates were added with 1 ml of each bu y coat platelet concentrates. The samples were split into ve groups: i-PRF, C-PRF, i-PRF metronidazole, C-PRF metronidazole, and control group. Then monitored after 1 day, 3 days, and 5 days using MTT assays. The data were inspected by Two Way ANOVA Test and Post Hoc Test. Results: The result indicated that cell proliferation of broblasts was escalated by the addition of C-PRF, i-PRF metronidazole incorporation, and C-PRF metronidazole incorporation group. While the i-PRF group showed decreasing proliferation cell count. Conclusion: This study assumed that platelet concentrates (i-PRF and C-PRF) with metronidazole incorporation can increase the cell proliferation higher than i-PRF and C-PRF only. © 2023, Universitas Hasanuddin Faculty of Dentistry. All rights reserved.