Evaluation of bio-compatibility and EffEctivEnEss of propolis TeTragonula sp. as dEntal anti-microbial agEnt

Adiningrat, Arya and Maulana, Ikhsan and Fadhlurrahman, Ahmad Ghitha and Kurniawan, Muhammad Fariez and Aji, Nur Rahman Ahmad Seno (2023) Evaluation of bio-compatibility and EffEctivEnEss of propolis TeTragonula sp. as dEntal anti-microbial agEnt. Journal of Stomatology, 76 (2). 94 – 100. ISSN 00114553

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Abstract

Introduction: Bacterial invasion is an important concern in dental therapy procedures. Oral bacteria, such as Enterococcus faecalis (E. faecalis) and Streptococcus mutans (S. mutans) could be more virulent in dysbiosis condition. Utilization of anti-microbial agent, e.g., chlorhexidine digluconate (CHX), is commonly applied for bacterial control in dental practice, since it harbors significant bacterial eradication. However, bio-compatibility concerns arise along with chemical anti-microbial agent towards surrounding tissues. Propolis, with its’ various bio-active compounds is reported to inhibit bacterial growth without artificial chemical substance, and making it as a promising alternative anti-microbial agent. Objectives: This study aimed to evaluate anti-bacterial capacity of the propolis compound and its’ cyto-toxic effects against human fibroblast. Material and methods: 0.1 and 1 of both ethanolic- (EEP) and water-based (WEP) compounds of local propolis were prepared prior to experimental procedures. Both E. faecalis ATCC 29212 and S. mutans ATCC 25175 were measured at 625 nm for McFarland’s initial adjustment, and turbidity at 600 nm for growth evaluation and antibacterial activity. Cyto-toxic effects of both extracts towards fibroblast cells were evaluated through cells viability test using MTS method. Shapiro-Wilk test was performed followed by ANOVA, t-test, or Mann-Whitney U-test. Results: It showed that the EEP tended to be more toxic than the WEP against human fibroblast cells in higher concentrations. Both 1 of WEP and EEP showed significant bacterial inhibitory activities against E. faecalis and S. mutans. Conclusions: These findings suggest the enhanced possibility of propolis-based compound as a promising dental anti-microbial agent. © 2023 Polish Dental Association.

Item Type: Article
Additional Information: Cited by: 1; All Open Access, Gold Open Access
Uncontrolled Keywords: Enterococcus faecalis; Streptococcus mutans; dysbiosis; water-based propolis; ethanolic-based propolis; bio-compatibility
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dental Study Program Academic Phase
Depositing User: Desy Natalia Anggorowati Anggorowati
Date Deposited: 09 Oct 2024 00:54
Last Modified: 09 Oct 2024 00:54
URI: https://ir.lib.ugm.ac.id/id/eprint/8386

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