Enzymatic activity of alkaline protease from bacillus cereus TD5B and its application as sheep skin dehairing agent; L’activité enzymatique de la protéase alcaline de bacillus cereus td5b et son application comme agent d'élimination des cheveux pour la peau de mouton; Activitatea enzimatică a proteazei alcaline din bacillus cereus TD5B și aplicarea acesteia pe pielea de oaie ca agent de îndepărtare a părului

This study aims to determine the enzymatic activity of extracellular alkaline protease from Bacillus cereus TD5B and its potential application as a sheep skin dehairing agent. The B. cereus TD5B was screened for extracellular alkaline protease production on skim milk agar media, while its alkaline protease activity and the application were measured at 1, 1.5, and 2. The application of alkaline protease from B. cereus TD5B as a sheep skin dehairing agent was observed through histological examination and physical properties measurement after chrome-tanning with lime and Na2S as control. The study was conducted in a completely randomized design, and the quantitative data were analyzed using Duncan’s Multiple Range Test. The results showed that a clear zone was seen surrounding B. cereus, indicating the bacteria’s proteolytic activity. The protease activity measurement showed that 2 of alkaline protease had the highest enzymatic activity at 144.75 U/mL/min. The highest tensile strength of sheep leather was obtained after dehairing at 1 alkaline protease concentration (350.26 kg/cm2), even though the highest elongation was obtained at 2 (34.92). In contrast, different concentrations showed similar shrinkage temperatures at 90°C. This study concludes that the optimum alkaline protease concentration from Bacillus cereus TD5B as a sheep dehairing agent was 2. © 2021 by the author(s). Published by INCDTP-ICPI, Bucharest, RO.