Sukirno, Sukirno and Sumarmi, Siti and Soesilohadi, R. C. Hidayat and Sudaryadi, Ign. and Purwanto, Hari and Aldawood, Abdulrahman Saad (2023) The Effects of Ultraviolet B on The Efficacy of Bacillus thuringiensis var. kurstaki Formulations Against Tobacco Armyworm, Spodoptera litura (Lepidoptera: Noctuidae). HAYATI Journal of Biosciences, 30 (1). pp. 17-27. ISSN 19783019
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Abstract
Tobacco armyworm (Spodoptera litura Fab.) is one of the major insect pests of crops in Indonesia. The management of this pest still depends on the use of chemical insecticides. The use of bio-insecticides, such as Bacillus thuringiensis (Bt.), are known to be alternatives, but it easily degraded by sunlight. This research aimed to study the effects of UV B on Bt. pathogenicity and to explore plant-based additives as UV B protectants for Bt. against armyworm. Thirteen plant extracts were screened based on their UV spectra absorbencies using UV spectrophotometry. The extracts, namely cloves, Jicama, Celebes pepper, turmeric, and Moringa, then used for the formulations of Bt. and exposed under UV B lights for 0, 72, and 144 h. After exposure to UV B, Bt. formulations were tested for bioassay against one-day-old 1st and one day old 2nd larval instars of tobacco armyworm. The result indicated that at 72 and 144 h of UV B exposures, the Bt. added formulations were signifcantly different compared to Bt. alone. At 72 h exposure against the 2nd larval instar, the larval mortality of tobacco armyworm on the second day of observation on Bt. formulated with Celebes pepper and turmeric was 97.3 and 80, respectively, whereas, at 144 h exposure, the mortality was 96 and 89.3, respectively. This study concluded that Celebes pepper and turmeric extracts were the potential to be used as the Bt. protectants against UV B. © 2023, Bogor Agricultural University. All rights reserved.
Item Type: | Article |
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Additional Information: | Cited by: 1; All Open Access, Gold Open Access |
Uncontrolled Keywords: | Bacillus thuringiensis, UV Protectant, armyworm, Indonesia |
Subjects: | Biology |
Divisions: | Faculty of Biology > Doctoral Program in Biology |
Depositing User: | Rusna Nur Aini Aini |
Date Deposited: | 03 Mar 2025 03:26 |
Last Modified: | 03 Mar 2025 03:26 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/9593 |