Nitrogen sources affect the growth of local strain Euglena sp. isolated from Dieng Peatland, Central Java, Indonesia, and their potential as bio-avtur

Euglena sp. is a member of the Euglena genus that has been successfully isolated under extreme conditions. The pH level of Dieng Peatland is 2.0-3.5. that suitable for the growth of Euglena. There are several ways to increase biofuel production in Euglena sp. by increasing the production of lipids and fatty acids produced through cultivation engineering, metabolic engineering and genetic engineering. One of the efforts in increasing growth, biomass and lipid levels in microalgae, especially Euglena sp. is to provide nitrogen variation in his cultivation medium. The concentration of nitrogen applied to the culture medium is able to increase growth, biomass production and total lipids in microalgae. The aim of this article was to analyze the effect of nitrogen sources in form of Sodium Nitrate (NaNO3) on the growth of microalgae and biomass production. The results showed that NaNO3 stimulated Euglena sp. to produce the highest amount of biomass (0,377 gram/L) after adding 1,5 grams of NaNO3. © 2023 American Institute of Physics Inc.. All rights reserved.