Attacus atlas (L.) sericin extract as an effective UV Protectant of Bacillus thuringiensis serotype kurstaki for controlling Spodoptera litura (Fab.)

Ningtyas, Nindita Sabila and Rahmatullah, Rahmatullah and Wiranto, Aryo Seto Pandu and Sa’adah, Nadya Sofia Siti and Alwandri, Hipny and Asma, Asma and Salsabila, Tiara Purti and Adi, Hanindyo and Sukirno, Sukirno (2023) Attacus atlas (L.) sericin extract as an effective UV Protectant of Bacillus thuringiensis serotype kurstaki for controlling Spodoptera litura (Fab.). Journal of Tropical Biodiversity and Biotechnology, 8 (1). pp. 1-9. ISSN 25409573

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Abstract

Bacillus thuringiensis serotype kurstaki is an entomopathogenic bacteria commonly used to control the cutworm Spodoptera litura (Fab.). However, B. thuringiensis has disadvantage of being easily degraded due to sunlight. The objective of this research was to determine the effectiveness of adding A. atlas (L.) cocoon extract as UV protectant B. thuringiensis to the mortality of S. litura. This research formulated 2.5 of the original substance of A. atlas cocoon extract and B. thuringiensis serotype kurstaki strain HD-7 applied from commercial product DiPel-WP®. The formulation was exposed to sunlight for 0, 1, 2, and 3 weeks. The suspension treated for 20 individuals of first instar larvae S. litura shifted into the artificial diet using 3-5 replicates. The scanning electron microscope (SEM) method began from a sample that was vacuumed, sample coated, and observed on SEM with the electron in a certain level probe. This research showed that the mortality of S. litura decreased with the growth of S. litura. The mortality of S. litura achieved 20-100 mortality after treatments. The A. atlas cocoon extract was effective as UV protectant B. thuringiensis for three weeks of exposure to sunlight. The SEM analysis represented that formulation of B. thuringiensis and A. atlas cocoon extract with sunlight exposure for one week has more rough surface than that of exposed during three weeks. Copyright: © 2023, J. Tropical Biodiversity Biotechnology.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access, Green Open Access
Uncontrolled Keywords: mortality, pathogenicity, SEM (Scanning Electron, Microscope) Spodoptera litura
Subjects: Biology
Divisions: Faculty of Biology > Doctoral Program in Biology
Depositing User: Rusna Nur Aini Aini
Date Deposited: 27 Feb 2025 04:19
Last Modified: 27 Feb 2025 04:19
URI: https://ir.lib.ugm.ac.id/id/eprint/9624

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