Entomological origin detection of honey from Apis mellifera and Apis cerana javana in Indonesia based on the Major Royal Jelly Protein 2 (mrjp2) gene

Raffiudin, Rika and Shullia, Nurul Insani and Febiriani, Tia Vina and Nisa, Nisfia Rakhmatun and Rahmadini, Jenny and Purwanto, Hari and Atmowidi, Tri (2021) Entomological origin detection of honey from Apis mellifera and Apis cerana javana in Indonesia based on the Major Royal Jelly Protein 2 (mrjp2) gene. Journal of Apicultural Research, 62 (2). 330 -333. ISSN 00218839

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Abstract

The entomological origin of honey was detected in the subtropical Apis cerana cerana using two sets of Major Royal Jelly Protein 2 (mrjp2) primers. Due to the high genetic divergence of A. cerana, this study aimed to confirm that the primers can be used to detect honey origin from A c. javana and Apis mellifera in Indonesia. The mrjp2 gene was amplified using M-F M-R and C-F C-R primers at various annealing temperatures to find the optimal temperature based on the presence-absence of the amplicon. Honey was successfully differentiated from 11 colonies of A. mellifera and A. c. javana using M-F and M-R primers at annealing temperatures of 50 °C, 53 °C, 55 °C, 57 °C, and 59 °C. However, caution should be placed on using these primers when used to amplify A. cerana at 47 °C. This study confirmed that C-F and C-R primers are specifically applicable when amplifying A. c. javana.

Item Type: Article
Additional Information: Library Dosen
Uncontrolled Keywords: Apis cerana; Apis mellifera;entomological detection;honey; honeyauthentication; Major RoyalJelly Protein 2 gene
Subjects: Biology
Divisions: Faculty of Biology > Doctoral Program in Biology
Depositing User: Rusna Nur Aini Aini
Date Deposited: 11 Dec 2024 04:01
Last Modified: 11 Dec 2024 04:01
URI: https://ir.lib.ugm.ac.id/id/eprint/9639

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