Cell line screening and characterization of bioactive compounds from kaffir lime (Citrus hystrix DC.) cell suspension elicited by yeast and isopentenyl pyrophosphate

Damayanti, Frisca and Rizqi, Dewi Y. and Mafiroh, Wulan U. and Salamah, Umi and Cristy, Ghea P. and Sasongko, Aries B. and Indrianto, Ari and Semiarti, Endang and Tunjung, Woro A.S. (2023) Cell line screening and characterization of bioactive compounds from kaffir lime (Citrus hystrix DC.) cell suspension elicited by yeast and isopentenyl pyrophosphate. ScienceAsia, 49 (4). pp. 507-514. ISSN 15131874

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Abstract

Elicitation and precursor feeding are two of available strategies for enhancing bioactive production in plant cell line screening. This study aimed to select the optimum kaffir lime cell line, which yields more bioactive constituents, using elicitation and precursor feeding and analyze its bioactive compounds. Firstly, we induced callus from the seed explant. The callus tissues were, then, subjected to the cell suspension. As much as 10 mg/ml of pure culture S. cerevisiae (SC), 5 mg/ml (F5), and 10 mg/ml (F10) of commercial technical-grade yeast were used as elicitors. Furthermore, 10 µg/ml of Isopentenyl pyrophosphate (IPP) was used as a precursor. After obtaining the cell lines, we analyzed the profile of the bioactive compounds by GC-MS. The results showed that the number of living kaffir lime cells after treatments decreased by 37.1 to 40.4, indicating a successful cell line selection. The resistant cells were assumed to be the cell lines capable of producing a higher concentration of target compounds (terpenoids). Bioactive compounds detected in all treatments were dominated by alkane, fatty acid, and aldehyde compounds. F5 and SC treatment enhanced more terpenoids compounds compared with untreated cells. It can be concluded that all treatments indicated a successful cell line selection, in which F5 and SC enhanced more terpenoids compared with the control. Terpenoid-producing cell lines can be used as a raw material for cancer medicine. © 2023 Science Society of Thailand under Royal Patronage. All rights reserved.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access
Uncontrolled Keywords: kaffir lime cell line, elicitor, precursor feedings, terpenoids, yeast
Subjects: Biology
Divisions: Faculty of Biology > Doctoral Program in Biology
Depositing User: Rusna Nur Aini Aini
Date Deposited: 25 Oct 2024 05:52
Last Modified: 25 Oct 2024 05:52
URI: https://ir.lib.ugm.ac.id/id/eprint/9649

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