The effect of 2,4-D, thidiazuron and BAP on calli induction of arabica coffee (Coffea arabica L.)

Arimarsetiowati, R. and Putra, A.C.D.S. and Suwastono, M.R. and Umami, L.A. and Daryono, B.S. and Astuti, Y.T.M. and Semiarti, E. (2023) The effect of 2,4-D, thidiazuron and BAP on calli induction of arabica coffee (Coffea arabica L.). In: International Conference on Modern and Sustainable Agriculture (ICOMSA), 2 Agustus 2022 - 3 Agustus 2022, IPB Bogor.

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Abstract

Four continents and numerous developing contries benefit economically from the annual income and jobs that coffee provides. The only polyploid species found in the genus Coffea is called Coffea arabica, and it belongs to the Rubiaceae family. In order to commercially propagate plants or to learn more about key concepts in somatic embryo induction and development, somatic embryogenesis is used. The purpose of this experiment was to compare how calli induction was impacted by several plant growth regulators in the Arabica coffee. Leaf tissues were cultivated on ½ MS medium with varying concentrations of 2,4-D and thidiazuron (1.0, 2.0, 3.0 mg/L) together with 1.0 mg/L BAP. Each experiment used a minimum of seven replicated abd was repeated three times using a completely random design. The varied grades of calli’s morphological examination was identified. Up to eight weeks, the proportion of calli development was measured every two weeks. Nearly sixty percent of calli formed in the medium of 1 mg/L 2,4D and 1 mg

Item Type: Conference or Workshop Item (Paper)
Additional Information: Cited by: 1; Conference name: International Conference on Modern and Sustainable Agriculture 2022; Conference date: 2 August 2022 through 3 August 2022; Conference code: 186217; All Open Access, Gold Open Access
Uncontrolled Keywords: arabica, auxin, embryo, calli, cytokinin.
Subjects: Biology
Divisions: Faculty of Biology > Doctoral Program in Biology
Depositing User: Rusna Nur Aini Aini
Date Deposited: 28 Oct 2024 07:06
Last Modified: 28 Oct 2024 07:06
URI: https://ir.lib.ugm.ac.id/id/eprint/9652

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