The Effect of Tofu Wastewater and pH on the Growth Kinetics and Biomass Composition of Euglena sp.

Media and pH are two crucial factors in microalgal cultivation. Industrial wastewater such as tofu wastewater can be utilized as alternative media for growing microalgae like Euglena sp. to produce biomass as feedstock in biorefinery activities. Here, we evaluated combinations of tofu wastewater (L) consisting of 0 (L1), 75 (L2), and 100 (L3) with pH (P) levels consisting of 5.0 (P1), 5.5 (P2), 6.0 (P3), 6.5 (P4), and 7.0 (P5). The analyses were carried out on the growth kinetics, biomass, primary metabolite compounds, and pigments of Euglena sp. Based on the study, the combinations with the highest cell density, biomass, maximum carbohydrate content, maximum lipid content, and protein content were L2P2 (23.13x105 cells/ml), L2P1 (4.53±0.17 mg/ml), L1P5 (0.93±0.02 mg/ml), L2P1 (1.27±0.11 mg/ml), and L3P4 (256±26.86 ppm), respectively. Moreover, the combinations with the highest chlorophyll-a, chlorophyll-b, and carotenoid were L2P4 (33.53±0.13 mg/l), L2P2 (17.73±0.50 mg/l), and L2P2 (11.65±0.00 mg/l), respect