Anatomical Studies and Evaluation of Genetic Stability in Plantlets Derived from Somatic Embryos of Arabica Coffee

Arimarsetiowati, Rina and Daryono, Budi Setiadi and Astuti, Yohana Theresia Maria and Semiarti, Endang (2023) Anatomical Studies and Evaluation of Genetic Stability in Plantlets Derived from Somatic Embryos of Arabica Coffee. HAYATI Journal of Biosciences, 30 (3). 510 -521. ISSN 19783019

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Abstract

Anatomical characteristics regenerant plantlet of Arabica coffee (Coffea arabica L.) were observed to determine the difference of plantlet performance between Sigararutang and Maragogige grown in shooting and rooting medium. Transverse sections of the fresh roots, stems and leaves of three-month-old plantlets from somatic embryos were collected and used for the study. Sigararutang and Maragogipe as the plantlet materials were chosen based on the bean size and the origin. Stomata were microscopically observed on the abaxial leaf paradermal section. A conformity test to compare between plantlet and the parent plant was observed to perform genetic stability. Assessment of genetic stability was measured by using the sequence of trnL (UAA) region. The result showed that all the anatomical roots, stems and leaves of the Maragogipe plantlet have a greater number than Sigararutang (root diameter, cortex thickness, distance of long stele, distance of short stele, endodermis thickness, stem diameter, cortex thickness,

Item Type: Article
Additional Information: Cited by: 1; All Open Access, Gold Open Access
Uncontrolled Keywords: natomy, Coffea arabica L., genetic variation,Maragogipe, Sigararutang, trnL* Corresponding AuthorE-mail Address: endsemi@ugm.ac.id Vol. 30 No. 3, May 2023 510-521DOI:10.4308/hjb.30.3.510-521ISSN: 1978-3019EISSN: 2086-4094H A Y AT IH A Y AT IJournal of BiosciencesJournal of Biosciences
Subjects: Biology
Divisions: Faculty of Biology > Doctoral Program in Biology
Depositing User: Rusna Nur Aini Aini
Date Deposited: 25 Oct 2024 03:24
Last Modified: 25 Oct 2024 03:24
URI: https://ir.lib.ugm.ac.id/id/eprint/9673

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