In vitro regeneration of hybrid Dendrobium sect. Spatulata through pseudobulb segment culture

The low rate of Dendrobium regeneration through seed culture is a significant limitation of mass propagation development in new hybrids. An efficient in vitro regeneration protocol through pseudobulb segment culture has been established for Dendrobium’Dandy Dame’. Leaves and roots of seven-month-old seedlings were detached from the pseudobulb. Unsegmented pseudobulb and segments of apical, medial, and basal excised from seedlings. The four types of explants were cultured on Murashige and Skoog (MS) basal medium supplemented with different combinations of 1-naphthaleneacetic acid (NAA) and 6-benzyl amino purine (BAP). The highest number of shoots was observed in 1 mg L-1 BAP and 0.1 mg L-1 NAA using unsegmented pseudobulb explant after two months of culture. The unsegmented pseudobulb had a higher survivability rate and the number of shoots per explant than the segmented type. However, segmented pseudobulbs can produce 2-4 times more shoots than unsegmented when accumulated. The basal segment had the highest a