Raffiudin, Rika and Shullia, Nurul Insani and Febiriani, Tia Vina and Nisa, Nisfia Rakhmatun and Rahmadini, Jenny and Purwanto, Hari and Atmowidi, Tri (2021) Entomological origin detection of honey from Apis mellifera and Apis cerana javana in Indonesia based on the Major Royal Jelly Protein 2 (mrjp2) gene. Journal of Apicultural Research, 62 (2). pp. 330-333. ISSN 00218839
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Abstract
The entomological origin of honey was detected in the subtropical Apis cerana cerana using two sets of Major Royal Jelly Protein 2 (mrjp2) primers. Due to the high genetic divergence of A. cerana, this study aimed to confirm that the primers can be used to detect honey origin from A c. javana and Apis mellifera in Indonesia. The mrjp2 gene was amplified using M-F M-R and C-F C-R primers at various annealing temperatures to find the optimal temperature based on the presence-absence of the amplicon. Honey was successfully differentiated from 11 colonies of A. mellifera and A. c. javana using M-F and M-R primers at annealing temperatures of 50 °C, 53 °C, 55 °C, 57 °C, and 59 °C. However, caution should be placed on using these primers when used to amplify A. cerana at 47 °C. This study confirmed that C-F and C-R primers are specifically applicable when amplifying A. c. javana. © 2021 International Bee Research Association.
Item Type: | Article |
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Additional Information: | Cited by: 5 |
Uncontrolled Keywords: | Apis cerana; Apis mellifera; entomological detection; honey; honey authentication; Major Royal Jelly Protein 2 gene |
Subjects: | Q Science > QH Natural history > QH301 Biology |
Divisions: | Faculty of Biology > Doctoral Program in Biology |
Depositing User: | Rusna Nur Aini Aini |
Date Deposited: | 15 Oct 2024 07:22 |
Last Modified: | 15 Oct 2024 07:22 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/9680 |