Ameliorative effect of Mangosteen (Garcinia mangostanaL.) peel infusion on the histopathological structures of the liver and kidney of rats (Rattus norvegicus Berkenhout, 1769) after H2O2 induction

Background and Aim: Hydrogen peroxide (H2O2) is a free radical, widely use as a food preservative, may cause adverse effects to the body. Mangosteen contains various antioxidants may scavenge free radical produced by H
2O2 . This study examined the effects of mangosteen peel infusion on the liver and kidney of rats after H
2O2 induction.

Materials and Methods: Thirty-six female Wistar rats were divided into six groups: Negative control, positive control
(1% H2O2), as well as mangosteen peel infusion groups at a concentration of 0.25; 0.5; 1, and 2%. H 2O2 induction was
administered orally every day for 2 months followed by mangosteen peel infusion treatment (1 month) after H2O2
induction for 1 month. At the end of the experiment, the rats were sacrificed. The liver and kidney of each rat were collected for reactive oxygen species (ROS) and histopathological examinations. Furthermore, blood was collected for creatinine examination.

Results: H2O2 induction caused the highest ROS level elevation in the positive control group which was treated with H2O2 only. Meanwhile, 2% of mangosteen peel infusion returned the ROS levels to normal. It was also observed that
mangosteen peel infusion restored creatinine levels to normal. Furthermore, 2% of mangosteen peel infusion improved the histopathological structure of the liver and kidneys after H 2O2 induction.

Conclusion: Mangosteen peel infusion especially at a concentration of 2% has the potential to improve liver and kidney structure and functions after H 2O2 induction.