Measurement of lipid droplets in peripheral immune cells shows an immunomodulatory effect on monocyte polarization in experimental dyslipidaemia

Hung, Yu-Ping and Chen, Chia-Ling and Tseng, Po-Chun and Satria, Rahmat Dani and Chen, Mei-Chieh and Lin, Chiou-Feng (2023) Measurement of lipid droplets in peripheral immune cells shows an immunomodulatory effect on monocyte polarization in experimental dyslipidaemia. Biochemical and Biophysical Research Communications, 650. 73 – 80. ISSN 0006291X

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Abstract

Intracellular lipid droplet (LD) generation is the primary site of energy storage, which is necessary for physiological homeostasis but is related to pathological metabolic disorders. Lipid metabolism is critical for maintaining innate and adaptive immunity; however, it is mainly undefined in peripheral immune cells. Flow cytometry-based immune profiling in healthy peripheral blood cells showed significant original generation of LDs in dendritic cells (DCs, CD3−CD19−CD56−CD11+), monocytes (CD3−CD19−CD56−CD14+), natural killer cells (NK, CD3−CD19−CD56+), and B cells (CD3−CD19+). CD36, a common scavenger receptor of lipids, was also highly expressed in LD-accumulated DCs and monocytes. Following short-term treatment with oxidized LDL (oxLDL) in an experimental ex vivo model, CD14+ monocytes showed an effective increase in LD generation, but there were no alterations in the immune cell populations. Furthermore, oxLDL-treated CD14+ monocytes displayed CD36 expression. However, oxLDL-primed CD14+ monocytes showed a blockade in the uptake of extra oxLDL, even while expressing increased CD36, indicating a defect in lipid clearance. Exogenous treatment with oxLDL caused monocyte type 1 polarization accompanied by increased LD accumulation and CD36 expression. This study describes a method to monitor LD generation and CD36 expression in peripheral immune cells and identified an immunomodulatory effect of oxLDL on monocytes by tilting them towards type 1 polarization. © 2023 Elsevier Inc.

Item Type: Article
Additional Information: Cited by: 1
Uncontrolled Keywords: CD36 Antigens; Dyslipidemias; Humans; Lipid Droplets; Lipoproteins, LDL; Monocytes; Receptors, Scavenger; CD11 antigen; CD14 antigen; CD19 antigen; CD3 antigen; CD36 antigen; CD56 antigen; cell surface marker; fat droplet; heparin; oxidized low density lipoprotein; fat droplet; low density lipoprotein; scavenger receptor; Article; cell isolation; controlled study; dendritic cell; dyslipidemia; ex vivo study; flow cytometry; fluorescence intensity; human; human cell; human tissue; immunocompetent cell; immunofluorescence; immunohistochemistry; immunomodulation; immunophenotyping; monocyte; natural killer cell; peripheral blood mononuclear cell; polarization; regulatory T lymphocyte; metabolism
Subjects: R Medicine > RB Pathology
Divisions: Faculty of Medicine, Public Health and Nursing > Biomedical Sciences
Depositing User: Sri JUNANDI
Date Deposited: 07 Nov 2024 01:06
Last Modified: 07 Nov 2024 01:06
URI: https://ir.lib.ugm.ac.id/id/eprint/10765

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