Transformation of HBcAg gene of hepatitis B virus using pEGFP-C1 vector

The HBcAg gene is a specific gene for the detection of hepatitis B virus (HBV). Antibodies against HBcAg are the most reactive among other antibodies. DNA transformation is the process of inserting DNA into a bacterial cell through the transfer of a plasmid which contains the genetic material. This study aims to evaluate the results of the transformation of the pEGFP-C1 plasmid containing the HBcAg gene. This research begins with molecular cloning of E. coli DH5α bacteria. E. coli DH5α was prepared to become competent cells. In addition, the plasmid containing the HBcAg gene was transformed into E. coli DH5α host cells by the heat shock method. Transformation was assessed by the presence of E. coli DH5α on LB agar containing the antibiotic kanamycin. Gene conjugated plasmids in E. coli DH5a were analyzed by 1 agarose gel electrophoresis. The results of this study indicated that the transfection of the pEGFP-C1 plasmid containing the HBcAg gene into E. coli DH5α was successfully carried out, marked by the growth of E. coli DH5α colonies. coli DH5α on LB agar containing the antibiotic kanamycin. The picture on the agarose gel shows that the plasmid carrying the HBcAg gene can be transformed into E. coli DH5α bacterial cells. © 2023 AIP Publishing LLC.