Hematological Profile of Mice After Ethyl Acetate Extract of Fungus Comb of Indo-Malayan Termite (Macrotermes gilvus Hagen) Mound Supplementation in Regulating Lipopolysaccharide-induced Inflammatory Response

Caesario, Jeferson and Hertanto, Decsa Medika and Susanto, Hermawan and Sudiana, Ketut and Nandika, Dodi and Karlinasari, Lina and Arinana, Arinana and Batubara, Irmanida and Witasari, Lucia Dhiantika and Rachmayanti, Yanti and Firmasyah, Dikhi and Santoso, Djoko (2023) Hematological Profile of Mice After Ethyl Acetate Extract of Fungus Comb of Indo-Malayan Termite (Macrotermes gilvus Hagen) Mound Supplementation in Regulating Lipopolysaccharide-induced Inflammatory Response. Malaysian Journal of Medicine and Health Sciences, 19. 57 – 63. ISSN 16758544

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Abstract

Introduction: Termitomyces sp. is an edible fungal species commonly found in tropical forests in Africa and Southeast Asia. It is consumed as an immunomodulatory agent to enhance the immune system. This study explored the hematological profile of ethyl acetate extract of fungus comb(EAEFC) on mice’s inflammatory response induced by intraperitoneally-injected lipopolysaccharide. Methods: An experimental study with a post-test-only control group design using BALB/C mice(n = 24, bodyweight/BW:25-30 grams) was conducted. The animals were randomly allocated into 4 groups, each consisting of 6 mice, and received substance(s) via gavage. Groups I and III received 5 Dimethyl Sulfoxide(DMSO) in water, groups II and IV received 500mg/kg BW EAEFC in 5 DMSO. On day 15, Group I and II were injected intraperitoneally with 5 ml/kg BW saline, whereas Group III and IV with 10 mg/kg BW lipopolysaccharide(LPS) in 5 ml/kg BW saline. After three hours, the mice were sacrificed; their blood was collected, and a hematological profile was observed. The results were displayed as mean ± standard deviation (SD), and the differences between groups were statistically analyzed using one-way ANOVA. The non-normally distributed data were analyzed using Kruskal-Wallis followed by Man-Whitney U test. Results: Peripheral blood study revealed that the levels of leukocyte count from the EAEFC group(group II) increased significantly when compared to the negative control(group I). Group IV had higher granulocyte and lower lymphocyte percentages than group III, suggesting the potential inflammation-regulating effect of EAEFC, while there is no marked difference in group I and II without LPS-induced inflammatory response. Conclusion: Results indicated that EAEFC altered leukocyte responses when the inflammation occurred. © 2023 UPM Press. All rights reserved.

Item Type: Article
Additional Information: Library Dosen
Uncontrolled Keywords: Termitomyces, ethyl acetate extract of fungus comb, lipopolysaccharide, inflammation, hematological profile
Subjects: S Agriculture > SD Forestry
Depositing User: Sri JUNANDI
Date Deposited: 14 Nov 2024 07:46
Last Modified: 14 Nov 2024 07:51
URI: https://ir.lib.ugm.ac.id/id/eprint/11076

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