Validation of a simple extraction procedure for stimulants identification from human plasma

In this study, a novel LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) method was designed using a simple extraction procedure that was scientifically developed to capture the most relevant stimulants, methamphetamine, MDA (3,4-Methylene​dioxy​amphetamine), MDMA (3,4-Methylenedioxy-methamphetamine), and MDEA (Methyl diethanolamine). The LC-MS/MS method was validated using US FDA (United States Food and Drug Administration) guidelines, and all validation requirements were satisfactory. This protein precipitation method was specifically developed for handling large numbers of samples with minimum cost and volume of sample. The developed method was accurate, precise, and reproducible for quantification of stimulant from human plasma. This pilot study enrolled 100 drug addicts to evaluate stimulant testing from plasma. Our findings found 47 cases with positive methamphetamine. This method may be valuable for stimulant blood screening for a large and varied population because of its efficiency and economical aspects. © 2020, Institute of Medico-Legal Publications. All rights reserved.