Reducing sugar production of 3 species mushrooms spent media for bioethanol

Using lignocellulose materials as bioethanol feed stocks requires an efficient pretreatment process to reduce cellulose resistance. Among various types of pretreatment, biological pretreatment is a low-cost pretreatment technique. The edible white-rot fungus is a promising candidate for pretreatment of lignocellulosic biomass. This research used media waste from 3 types of edible mushrooms namely Ear, Lingzhi, and Oyster which has been cultivated for 90, 100, 110 days. The experiment results showed that the cultivation process can decrease the media weight up to 24.22% as well as reduce the content of chemical media components significantly. The interaction between the types of fungi and cultivation period provides a significant interaction on the extractive levels of ethanol-toluene, holocellulose, α-cellulose, hemicellulose, and acid soluble lignin. A hydrolysis rate of cultivation media waste on acid hydrolysis method is 0.28-28.7% while enzyme hydrolysis 9.3-25.64%. The results of t-test analysis between acid hydrolysis method and an enzyme showed significant difference in reducing sugar content. The average yield of reducing sugar levels of acid hydrolysis method (1.18 mg/ml) is lower than reducing sugar levels of enzyme hydrolysis method (3.5 mg/ml). The highest reducing sugar content was produced from Lingzhi mushroom waste media with cultivation period of 100 days and enzyme hydrolysis method, which amounted to 4.34 mg/ml. © 2020 Author(s).