Ahmad, Nazir and Lesa, Kaisun Nesa and Ujiantari, Navista Sri Octa and Sudarmanto, Ari and Fakhrudin, Nanang and Ikawati, Zullies (2024) Development of White Cabbage, Coffee, and Red Onion Extracts as Natural Phosphodiesterase-4B (PDE4B) Inhibitors for Cognitive Dysfunction: In Vitro and in Silico Studies. Advances in Pharmacological and Pharmaceutical Sciences, 2024. ISSN 26334682
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Human cognition fundamentally depends on memory. Alzheimer's disease exhibits a strong correlation with a decline in this factor. Phosphodiesterase-4 B (PDE4B) plays a crucial role in neurodegenerative disorders, and its inhibition is one of the promising approaches for memory enhancement. This study aimed to identify secondary metabolites in white cabbage, coffee, and red onion extracts and identify their molecular interaction with PDE4B by in silico and in vitro experiments. Crushed white cabbage and red onion were macerated separately with ethanol to yield respective extracts, and ground coffee was boiled with water to produce aqueous extract. Thin layer chromatography (TLC)-densitometry was used to examine the phytochemicals present in white cabbage, coffee, and red onion extracts. Molecular docking studies were performed to know the interaction of test compounds with PDE4B. TLC-densitometry analysis showed that chlorogenic acid and quercetin were detected as major compounds in coffee and red onion extracts, respectively. In silico studies revealed that alpha-tocopherol (binding free energy (ΔGbind) = -38.00 kcal/mol) has the strongest interaction with PDE4B whereas chlorogenic acid (ΔGbind = -21.50 kcal/mol) and quercetin (ΔGbind = -17.25 kcal/mol) exhibited moderate interaction. In vitro assay showed that the combination extracts (cabbage, coffee, and red onion) had a stronger activity (half-maximal inhibitory concentration (IC50) = 0.12 ± 0.03 μM) than combination standards (sinigrin, chlorogenic acid, and quercetin) (IC50 = 0.17 ± 0.03 μM) and rolipram (IC50 = 0.15 ± 0.008 μM). Thus, the combination extracts are a promising cognitive enhancer by blocking PDE4B activity. © 2024 Nazir Ahmad et al.
Item Type: | Article |
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Additional Information: | Cited by: 6; All Open Access, Gold Open Access, Green Open Access |
Uncontrolled Keywords: | alpha tocopherol; chlorogenic acid; coffee extract; nootropic agent; phosphodiesterase 4b; phosphodiesterase IV; plant extract; quercetin; red onion extract; unclassified drug; white cabbage extract; Alzheimer disease; Article; coffee; cognitive defect; computer model; densitometry; enzyme inhibition; IC50; in vitro study; molecular docking; molecular interaction; neuroprotection; nonhuman; onion; pharmacokinetic parameters; thin layer chromatography; white cabbage |
Subjects: | R Medicine > RS Pharmacy and materia medica |
Divisions: | Faculty of Pharmacy |
Depositing User: | Muh Aly Mubarok |
Date Deposited: | 03 Jul 2025 00:34 |
Last Modified: | 03 Jul 2025 00:34 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/19214 |